Mapping of promoter sites utilized by T3 RNA polymerase on T3 DNA
نویسندگان
چکیده
منابع مشابه
Physical mapping of hybrid bacteriophage T7/T3 RNA polymerase genes.
The late regions of the T7 and T3 bacteriophage genomes are transcribed by phage-specified RNA polymerases, the products of gene 1. Although these phage transcriptional systems share many characteristics and are obviously related, they have diverged to such an extent that neither of their respective RNA polymerases utilizes the promotor sites of the other phage at an appreciable rate. However, ...
متن کاملT7- and T3-specific RNA polymerases: characterization and mapping of the in vitro transcripts read from T3 DNA.
The late genetic region of the T3 bacteriophage genome is transcribed by a phage-specified RNA polymerase, the product of T3 gene 1. In vitro, purified T3 RNA polymerase acting with T3 DNA template synthesizes six major RNA products, with molecular weights of 5.5 X 10(6), 4.2 X 10(6), 1.7 X 10(6), 0.87 X 10(6), 0.52 X 10(6), and 0.23 X 10(6). These are designated T3 RNA species I through VI, re...
متن کاملIsolation and sequence determination of 5'-terminal oligonucleotide fragments of RNA transcripts synthesized by bacteriophage T3-induced RNA polymerase from T3 DNA.
The nucleotide sequence of the 5'-terminal oligonucleotides produced by pancreatic RNase digestion of bacteriophage T3 RNA polymerase (EC 2.7.7.6) transcripts of T3 DNA has been determined. The sequence determination is based upon a simple isolation procedure for the 5'-terminal oligonucleotides. This procedure involves treatment of pancreatic RNase digests of alpha 32P-labeled T3 RNA polymeras...
متن کاملLocation, function, and nucleotide sequence of a promoter for bacteriophage T3 RNA polymerase.
The major promoters for bacteriophage T3 RNA polymerase on the T3 genome have been mapped by DNA.RNA filter hybridization. One promoter is located in a 300-base-pair Hpa I restriction fragment near the genetic "left" end of T3 DNA. The sequence in the vicinity of the major initiation site of transcription in this region has been determined. A part of the (-)strand sequence is 5' T-A-T-T-T-A-C-C...
متن کاملIdentification of specific contacts in T3 RNA polymerase-promoter interactions: kinetic analysis using small synthetic promoters.
The T7, T3, and SP6 RNA polymerases recognize very similar, yet distinct, promoter sequences. The high homology among the promoter sequences suggests that differential promoter recognition must derive from relatively small changes in the protein. Steady-state kinetic analyses of transcription from the T3 consensus promoter and from promoters modified in the region critical to specific recogniti...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1980
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/8.21.5071